Education in Microscopy and Digital Imaging

Many of the speed limitations associated with point-scanning confocal microscopes can be overcome by imaging the specimen with multiple excitation beams operating in parallel. Spinning disk confocal microscopes are emerging as a powerful tool for rapid spatial and temporal imaging of living cells. Although the technique was originally introduced over 40 years ago, recent improvements in microscope optical design and camera technology have significantly expanded the versatility and potential of this approach. Among the most important aspects to understand about spinning disk confocal microscopy is that the these instruments are capable of acquiring thin optical sections from specimens in a manner similar to laser scanning confocal microscopes, only much faster. The digital videos in this gallery were acquired using a Yokogawa CSU-X1 spinning disk unit coupled to a ZEISS Axio Observer.

Grey Fox Lung Cells mEmerald-Actin and mRuby-LAMP1	Grey Fox Lung Cells mEmerald-α-Actinin and mApple-LAMP1	Grey Fox Lung Cells mEmerald-α-Actinin and mCherry-Mito	Grey Fox Lung Cells mEmerald-α-Actinin and mCherry-PDHA1	Grey Fox Lung Cells mEmerald-Fascin
Grey Fox Lung Cells mEmerald-Golgi and mCherry-PDHA1	Grey Fox Lung Cells mEmerald-MAP4 and mApple-H2B	Grey Fox Lung Cells mEmerald-MAP4	Grey Fox Lung Cells mEmerald-MAP4 and mApple-H2B	Grey Fox Lung Cells mEmerald-MAP4 and mCherry-Rab5a
Grey Fox Lung Cells mEmerald-Mito and mApple-α-Actinin	Grey Fox Lung Cells mEmerald-Mito and mApple-Golgi	Grey Fox Lung Cells mEmerald-PMP	Grey Fox Lung Cells mEmerald-VASP and mApple-LC Myosin	Grey Fox Lung Cells mRuby-Actin
Grey Fox Lung Cells mRuby-α-Actinin	Grey Fox Lung Cells mRuby-EB3	Grey Fox Lung Cells mRuby-CAAX	Grey Fox Lung Cells mRuby-LC Myosin	Grey Fox Lung Cells mTag-GFP2 and Paxillin
HeLa Cells mEmerald-Plastin	HeLa Cells mEmerald-PMP	HeLa Cells mRuby-Clathrin	HeLa Cells mRuby-CX26	HeLa Cells mTag-GFP2 and Connexin CX43
HeLa Cells mTag-GFP2 and Rab5a	Monkey Kidney Cells mEGFP-TFR and mCherry-CAAX	Monkey Kidney Cells mEmerald-Actin and mApple-LAMP1	Monkey Kidney Cells mEmerald-α-Actinin and mCherry-LAMP1	Monkey Kidney Cells mEmerald-α-Actinin and mCherry-PDHA1
Monkey Kidney Cells mEmerald-ER and mCherry-CAAX	Monkey Kidney Cells mEmerald-LifeAct and mCherry-LAMP1	Monkey Kidney Cells mEmerald-LAMP1 and mApple-α-Actinin	Monkey Kidney Cells mEmerald-Plastin and mCherry-Rab7a	Monkey Kidney Cells mEmerald-Plastin and mRuby-LAMP1
Monkey Kidney Cells mEmerald-PMP	Monkey Kidney Cells mEmerald-PMP and mApple-EB3	Monkey Kidney Cells mEmerald-PMP and mApple-ER-5	Monkey Kidney Cells mEmerald-PMP and mCherry-CAAX	Monkey Kidney Cells mEmerald-PMP and mRuby-ER
Monkey Kidney Cells mEmerald-α-Actinin and mCherry-PDHA1	Monkey Kidney Cells mEmerald-ER and mCherry-CAAX	Monkey Kidney Cells mEmerald-MAP4 and mCherry-H2B	Monkey Kidney Cells mEmerald MAP4 and mCherry-Rab5a	Monkey Kidney Cells mEmerald-MCAK
Monkey Kidney Cells mEmerald-PST and mCherry-C-Src	Monkey Kidney Cells mEmerald-PMP	Monkey Kidney Cells mEmerald-PMP and mApple-EB3	Monkey Kidney Cells mEmerald-PMP and mApple-ER-5	Monkey Kidney Cells mEmerald-PMP and mCherry-CAAX
Monkey Kidney Cells mEmerald-PMP and mRuby-ER	Monkey Kidney Cells mEmerald-Tubulin-18	Monkey Kidney Cells mEmerald-Tubulin-18 and mCherry-Rab11a	Monkey Kidney Cells mEmerald-Tubulin and mCherry-Rab5a	Monkey Kidney Cells mEmerald-Tubulin and mCherry-Rab7a
Monkey Kidney Cells mEmerald-Tubulin and mCherry-Rab11a	Monkey Kidney Cells mRuby-Actin	Monkey Kidney Cells mRuby-Endosomes	Monkey Kidney Cells mRuby-LC Myosin	Monkey Kidney Cells mRuby-LAMP1
Monkey Kidney Cells mTag-GFP2 and Tubulin	Mouse Melanoma mEmerald-Fascin and mCherry-H2B	Osteosarcoma mEmerald-α-Actinin and mCherry-CAAX	Osteosarcoma mEmerald-α-Actinin and mCherry-PDHA1	Osteosarcoma mEmerald-MAP4
Osteosarcoma mEmerald-MAP4 and mCherry-H2B	Osteosarcoma mEmerald MAP4 and mCherry-Rab5a	Osteosarcoma mEmerald-MAP4 and mApple-H2B	Osteosarcoma mEmerald-MAP4 and mCherry-H2B	Osteosarcoma mEmerald-MAP4 and mCherry-Rab5a
Osteosarcoma mEmerald-PMP	Osteosarcoma mEmerald-PMP and mApple-ER-5	Osteosarcoma mEmerald-PMP and mRuby-ER	Osteosarcoma mEmerald-Tubulin-18	Osteosarcoma mRuby-ER
Osteosarcoma mRuby-LC Myosin	Osteosarcoma mTag-GFP2-Actin	Pig Kidney Cells mEmerald-C-Src and mCherry-H2B	Pig Kidney Cells mEmerald-EB3 and mCherry-H2B	Pig Kidney Cells mEmerald-ER and mCherry-Rab7a
Pig Kidney Cells mEmerald-Fascin and mCherry-H2B	Pig Kidney Cells mEmerald-Golgi and mCherry-H2B	Pig Kidney Cells mEmerald-MAP4 and mCherry-Rab5a	Pig Kidney Cells mEmerald-Plastin	Pig Kidney Cells mEmerald-PMP and mApple-ER-5
Pig Kidney Cells mTag-GFP2 and Mito	Rabbit Kidney Cells mEmerald-Actin and mApple-LAMP1	Rabbit Kidney Cells mEmerald-Actin mApple-Paxillin	Rabbit Kidney Cells mEmerald-α-Actinin and mApple-LAMP1	Rabbit Kidney Cells mEmerald-MAP4 and mCherry-H2B
Rabbit Kidney Cells mEmerald-MAP4 and mApple-H2B	Rabbit Kidney Cells mEmerald-Plastin	Rabbit Kidney Cells mEmerald-Plastin and mApple-Paxillin	Rabbit Kidney Cells mEmerald-Plastin and mCherry-H2B	Embryonic Carcinoma mEmerald-Plastin and mApple-Paxillin