Pig Kidney Cells with mEmerald-C-Src and mCherry-H2B
In 1979, H. E. Varmus and J. M. Bishop's discovery that normal chickens contain a gene that is structurally closely-related to V-src called C-src completely changed the current thinking about cancer. The previously believed model where cancer is triggered by a viral gene was replaced with one in which a gene that is ordinarily present in the cell can cause cancer. Cultured pig kidney epithelial cells (LLC-PK1 line) appear in this digital video sequence with mEmerald fused to C-SRC and mCherry fused to histone H2B.
The green fluorescent protein mEmerald is a variant of EGFP that exhibits peak excitation and emission at 487 and 509 nanometers, respectively. Besides possessing all of the same mutations found in EGFP, mEmerald features four additional point mutations. The red fluorescent protein mCherry was developed through the directed evolution of mRFP1, an engineered mutant of Discosoma striata RFP. Excitation and emission of mCherry peak at 587 and 610 nanometers, respectively.